Ca was measured by methyl thymol blue method, P was determined with ultraviolet spectrophotography, determination of Mg and Cl were made with enzyme method, ALP was examined with 4 nitrobenzene phosphate method. 血清Ca2+测定用甲基麝香草酚蓝比色法,P3+测定用紫外分光光度法,Mg2+和Cl-测定用酶法,血清碱性磷酸酶测定用对-硝基苯磷酸盐比色法。